مقدمة في المعلوماتية الحيوية

Apply the previous steps in the video on the TNF gene in Mus musculus, searching in the Gene database, we need the results to include the
annotated genes and coding-proteins

we need the results to include the annotated genes and coding-proteins

1) Get the first 3 results, showing the synonyms of this gene, and their locations using the Genome viewer.

2) Try to find out if any phenotypes or genetic diseases are known to be associated with this gene in man.

3) Then pick up one of the related published articles of this gene and summarize its introduction part in your own writing style.

(In this field it is important to be familiar with the scientific community and be up-to-date by reading their work).

The catalase (Cat) gene is present in almost all aerobically respiring organisms, and protects cells from the toxic effects of hydrogen peroxide. It can also promote cell growth.
Use the current version of Ensembl to search for this gene in human, and answer the following questions:

How many transcripts does this gene have?

From the filtration section, navigate the molecular function of this gene, the variant table, and the gene expression, then interpret the first 3 results that you got from each term ( you may use Google to understand their concepts, then write down whatever you got from your total search).

The human ‘furry homolog (Drosophila)‘ gene (or FRY) is involved in cell morphogenesis, regulating the actin cytoskeleton, and patterning sensory neuron dendritic fields

You want to design primers to amplify the human FRY gene and understand a little bit about the genomic region.

Retrieve the nucleotide FASTA sequence from NCBI

Design your primers using Primer-BLAST

Follow the primer validation and PCR primer properties to make your decision, then mention which primers you selected and why?

Use Protein BLAST to search for the Monkeypox virus (MPXV) which is the causative agent of smallpox.

Use UniProt to get the MPXV in monkeypox organism, download the protein sequence in a FASTA format

Then, blast this sequence keeping the default parameters.

Select the top 5 results from your perspective and mention why you select them?

Identify the closely related viruses or strains of Monkeypox

I have attached a pdf file for this project